Cell-permeant and water-soluble rhodamine dyes for quantitative imaging applications

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Rhodamines are a class of fluorescent proteins (FP) that stand out for their higher photostability and brightness as well as easier synthesis compared to other FPs; however, classic rhodamines generally have poor water solubility and tend to aggregate in an aqueous environment. Oregon Health & Science University researchers have developed a water-soluble and cell-permeant rhodamine dye for improved quantitative imaging.

Technology Overview

Fluorescent proteins have become common optogenetic tools for investigating cell structures, functions, and underlying mechanisms of subcellular events. However, classic small molecule fluorophores often suffer from either poor water solubility or cell-impermeant issues, providing roadblocks for in vitro and in vivo optical imaging applications. OHSU researcher Dr. Summer Gibbs and colleagues have developed zwitterionic rhodamine fluorophores, which demonstrate several advantages over existing rhodamines and FPs:

•       Water permeable, thereby improving utility for applications that require aqueous environments, such as antibody conjugation and in vivo imaging

•       Cell permeable, allowing for targeting of both intracellular and extracellular targets

•       Lack of on-off equilibrium switching, providing improved utility for quantitative fluorescence imaging applications such as drug uptake and cellular distribution studies

These fluorophores have significantly improved optical properties for both in vitro and in vivo studies and represent a powerful new fluorescent dye for research and diagnostic purposes.

Licensing Opportunity

This technology is available for licensing.

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For Information, Contact:
Anne Carlson
Assoc Dir, Tech Dev & Licensing
Oregon Health & Science University
Summer Dowell (Gibbs)
Lei Wang
Antonio Montano
Imaging - Other
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Research Tools - Reagents
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