Mouse CD74: Design, codon optimization, cloning, expression in E coli, and purification

Case ID:
2187
Web Published:
3/1/2016
Description:

Protein: Recombinant mouse CD74

 

This recombinant mouse CD74 is a 191 amino acid residue polypeptide that lacks the C-terminal thyroglobulin domain. It is an E. coli expressed mouse protein that includes the intracellular domain (N-terminal amino acid residues 1 to 30), the transmembrane domain (amino acid residues 31 to 55) and the extracellular domain (amino acid residues 56 to 191). This protein would be useful for studies aimed at exploring protein-protein interactions, for blocking experiments of the MIF cytokine activity and could be useful to explore its interaction with other proteins that have been suggested to bind CD74.

 

Applications: Available as a research reagent, the protein can be used for protein-protein interactions; blocking experiments of MIF (macrophage inhibitory factor) cytokine activity; and interactions with other proteins that have been suggested to bind CD74.

 

Biological activity: This protein has been tested for its ability to bind MIF1 (or MIF), for its ability to bind several monoclonal antibodies currently used in CD74 biology research and for its capabilities to bind to recombinant human and mouse Class II proteins. Antibody-immobilized CD74 has been tested and shown to bind MIF in an in vitro assay.

 

Stability: When stored in low non-ionic detergent with 15% glycerol at -80˚C this protein is stable for at least 6 months with no appreciable degradation or reduction in its biological activity.

 

 

Patent Information:
For Information, Contact:
Michele Gunness
Senior Technology Development Manager
Oregon Health & Science University
(503) 494-8200
gunnessm@ohsu.edu
Inventors:
Roberto Meza-Romero
Arthur Vandenbark
Gil Benedek
Keywords:
Biological Materials
Biological Materials - Protein/Peptides
Research Tools
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