Spatially-resolved transcriptomics with enhanced capture and versatility

Summary
An improved platform for spatial transcriptomics, which could be mass produced and would allow for overall increased detection of transcripts, including for lower-expressed sequences.

Technology Overview
Spatial transcriptomics allows for the measurement of gene activity within a sample while mapping where that activity is occurring; however, current methods are limited in the number of probes that can fit onto the 2D sampling surface (i.e. slide).

OHSU researchers have developed an improved platform that allows for an increased number of probes and thus captured sequences. Features of this platform include:

• Projection of 2D array into 3D matrix substrate, like a hydrogel, increasing the capacity for spatially-captured barcodes across the thickness (z-plane) of the substrate.
• Material capture by electrophoresis, improving capture efficiency and limiting diffusion for better accuracy and resolution in spatial transcriptomics.
• Optional depletion step, that allows for the removal of house-keeping transcripts to improve detection sensitivity of lower-expressed sequences of interest.
• Compatible with current electrophoretic platforms, for easier adoption by end-users.
• Platform components are suitable for mass production.

Licensing Opportunity
This technology is available for licensing.