Sensor for Akt kinase activity in living cells

Case ID:
2049
Web Published:
10/8/2015
Description:

Technology Overview

Researchers at Oregon Health & Science University have developed a novel Akt biosensor which provides a robust readout for the PI3-kinase signaling pathway in living cells without the use complex microscopy techniques. This biosensor allows quantitative tracking of Akt activity for days in individual cells within a population, and responds within minutes to changes in the signaling pathway that is altered in a variety of disease states and physiological conditions. The biosensor provides a greater dynamic range, is more sensitive and more specific to changes in Akt activity than other FRET reporters, and does not require multiple fluorescent channels thus allowing for a second fluorescent reporter to be used for orthogonal monitoring of biochemical activity.

Publication

Gross SM, Rotwein P. Akt signaling dynamics in individual cells. J Cell Sci. 2015 Jul 15;128(14):2509-19. Link

Licensing Opportunity

Biological materials are available for non-exclusive licensing.

Patent Information:
Category(s):
Research Tools
For Information, Contact:
Trina Voss
Senior Technology Development Manager
Oregon Health & Science University
503-494-8200
vosst@ohsu.edu
Inventors:
Peter Rotwein
Sean Gross
Keywords:
Research Tools - Reagents
Research Tools - Screening
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