Hydrogen sulfide is an important biological signalling molecule and an important environmental detection target. Biological H2S exhibits significant effects in both the cardiovascular and neuronal systems and is produced endogenously from cystathionine-β-synthase (CBS), cystathionine-gamma-lyase (CSE), and 3-mercaptopyruvate sulfur transferase (3MST), as well as from non-enzymatic processes. Additionally, H2S is produced by many pathogenic bacteria and has been identified as a potential marker for pathogenic bacterial contamination of potable water sources in remote and developing areas. The Pluth lab has developed two methods for visual H2S detection, both of which allow for either naked-eye qualitative (color-change) observation or analytical quantification using common methods.