A strong micro-promoter for AAV

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Viral mediated transgene delivery often utilizes specialized promoters that drive ubiquitous expression; however, these promoters are large and limit the amount of remaining space in AAV vectors for the transgene. The laboratory of Dr. Markus Grompe at OHSU has identified a novel promoter, which drives strong expression in multiple cell types and is roughly one tenth the size of currently used promoters, thereby allowing for delivery of larger transgenes.

Technology Overview

Promoters such as CMV and CAG are used to drive strong ubiquitous expression of transgenes, but these promoters take up a large portion (~1 kb) of the available packaging space (4.8 kb) within AAV vectors. Oregon Health & Science University (OHSU) researchers have identified a novel ubiquitous promoter that is a fraction of the size of currently used promoters at only 84 bp. This promoter drives high expression, comparable to CMV and CAG promoters, in multiple mammalian cell types including human pancreatic endocrine cells and primary hepatocytes in vitro, and murine liver, muscle and brain in vivo. The considerable reduction in promoter size frees up valuable space for larger transgenes to be delivered by AAV vectors and could improve transduction efficiency. Utilization of this novel micro-promoter will allow for AAV delivery of a wider variety of transgenes to facilitate basic research and therapeutic development.

Licensing Opportunity

This technology is available for licensing.

Patent Information:
For Information, Contact:
Lisa Lukaesko
Technology Development Manager
Oregon Health & Science University
Markus Grompe
Sunghee Chai
Biological Materials
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