pLXSN human KIT V654A + D816Y

Case ID:
1658-E
Web Published:
1/29/2015
Description:

Technology Overview
This line was created by ENU-mutagenesis of BaF3 pLXSN KIT D816Y cell line and drug selection. The V654A mutation is associated with imatinib-resistance in GIST. This cell line expresses high levels of activated KIT. In addition to the engineered D816Y mutation, the cells also acquired the following mutations due to retrovirus recombination events: N587S and deletion of amino acids 931-961. These secondary events do not appear to alter the kinase properties of KIT or alter the pattern of D816Y sensitivity/resistance to kinase inhibitors. This cell line is strongly resistant to imatinib.

Patent Information:
For Information, Contact:
Trina Voss
Technology Development Manager
Oregon Health & Science University
4-8200
vosst@ohsu.edu
Inventors:
Michael Heinrich
Keywords:
Biological Materials
Biological Materials - Cell Lines
Research Tools
Research Tools - Screening
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