ARAC-8D cell line

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Technology Overview:

Two nucleoside transport-deficient cell lines were developed through isolation from a hypoxanthine-guanine phosphoribosyltransferase-deficient derivative of wild type CEM cells (a human T lymphoblast cell line originally derived from a patient with acute lymphocytic leukemia).  The Ara-C/8C and TUB-4D clones were isolated by virtue of their resistance to 8 µM arabinosylcytosine and 4µM tubercidin, respectively.  One cell line completely deficient in deoxycytidine kinase activity, the Ara-C/8D clone, is a clonal derivative of an hypoxanthine-guanine phosphoribosyltransferase-deficient parent CEM cell line originally isolated in semi-solid agarose containing 8µM arabinosylcytosine.  The Ara-C/8C and TUB-4D cell lines are cross-resistant to a spectrum of cytotoxic nucleosides to which parental and Ara-C/8D cell are sensitive. 

The Arac-C/8D cell line has been studied in detail for some time and found to express negligible deoxycytidine kinase activity and increased resistance to 2’-deoxyadenosine and 2’-deoxyguanosine-induced cytotoxicity as well as marked resistance to dideoxycytidine.  The Ara-C/8D cell line has been found to contain two DNA mutations within the 2’-deoxycytidein kinase gene conferring its resistance. 

Links/Publications:  Ulllman et al., J Biol Chem. 1988 Sep 5;263(25):12391-6.  Owens et al., Cancer Res. 1992 May 1;52(9):2389-93.

Licensing Opportunity:  The cell lines are available for non-exclusive licensing.


Patent Information:
Biological Materials
For Information, Contact:
Trina Voss
Technology Development Manager
Oregon Health & Science University
Buddy Ullman
Biological Materials
Biological Materials - Cell Lines
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