Mammalian cell lines expressing stably transfected somatostatin receptor SSTR1

Case ID:
0355-A
Web Published:
2/10/2010
Description:

Technology Overview

Technology 0355-A describes somatostatin which exerts multiple effects throughout the body by binding to specific somatostatin receptors. Two classes of somatostatin receptors (SRIF1 and SRIF2) had been distinguished biochemically and physiologically. cDNAs for two different rat somatostatin receptors have been characterized, inserted into expression vectors, and each stably transfected into CHO-K1 cells. The cell lines, expressing either SSTR1 or SSTR2, but not both, respond physiologically to somatostatin by inhibition of adenylyl cyclase activity by a pertusis-sensitive pathway. The rat somatostatin receptors expressed by these CHO cell lines are nearly identical to the human receptors, and appear physiologically indistinguishable from their human homologues.

 

Drug Screening

The cell lines can be used to screen for somatostatin agonists or antagonists, hopefully specific for either SSTR1 or SSTR2 types of receptors.  The cell lines can also be used in characterization of binding profiles of chug candidate molecules.

 

Related Publication
The Somatostatin Receptors SSTR1 and SSTR2 Are Coupled to Inhibition of Adenylyl Cyclase in Chinese Hamster Ovary Cells via Pertusus Toxin-Sensitive Pathways.  RE Herschenberger, BL Newman, T Florio,J Bunzow, O Civelli, X-J Li, M Forte & PJS Stork Endocrinology 134:1277-1285 (1994).


 
  
Patent Information:
Category(s):
Biological Materials
For Information, Contact:
Trina Voss
Technology Development Manager
Oregon Health & Science University
503-494-8200
vosst@ohsu.edu
Inventors:
Philip Stork
Michael Forte
Keywords:
Biological Materials
Biological Materials - Cell Lines
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